Primer Design

These are useful tools for primer design for PCR!

Calculate melting temperature

SimpleBio.dnatm — Function

dnatm(dnaseq::String)

Calculate the melting temperature of imput DNA sequence. The calculation is assume the annealing occurs under the standard conditions of 50 nM primer, 50 mM Na+, and pH 7.0.

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SimpleBio.step_gen — Function

step_gen(seq::String)

Generate a vector that contains stepwise increasing characters from the first character to the end.

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SimpleBio.step_from_end — Function

step_from_end(seq::String)

The reverse version of step_gen()

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SimpleBio.multidnatm — Function

multidnatm(dnas_array::Vector)

Generate a dataframe that shows all the Tm of sequences in the input array.

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SimpleBio.fwd_primers — Function

fwd_primers(seq::String)

Auto generation of foward primer candidates of an input sequence.

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fwd_primers(seq::String, tm_low::Int64, tm_high::Int64)

Auto generation of foward primer candidates of an input sequence in setted Tm range.

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SimpleBio.rev_primers — Function

rev_primers(seq::String)

Auto generation of foward primer candidates of an input sequence.

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rev_primers(seq::String, tm_low::Int64, tm_high::Int64)

Auto generation of foward primer candidates of an input sequence in setted Tm range.

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